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As the ground features are affected by various factors, the problem of endmember variability will occur. Endmember variability greatly affects the accuracy of spectral unmixing results. This study is based on the vegetation-soil binary scene, spectral unmixing is performed under the framework of NMF(Non-negative Matrix Factorization). The PROSAIL model is used to describe the variability of vegetation endmembers from the mechanism, so that the results of spectral unmixing have clear physical meaning. In order to improve efficiency, we setup two neural networks for model calculation and model inversion. In this way, the spectral unmixing algorithm can obtain the endmembers of the vegetation pixel by pixel, which can more accurately describe the variability of vegetation endmember. In addition, due to the diversity of spatial resolution of remote sensing data products. The problem of scale effect is widespread, which is a key issue in the field of remote sensing. Analysis of the reason is largely due to the mixed pixels of universally exist. Because of the method studied in this paper describes the variability of vegetation endmember. A spectral unmixing algorithm that can describe the variability of vegetation endmembers pixel by pixel is obtained. This result can be used to invert vegetation parameters. Therefore, this study attempts to correct the scale effect of remote sensing products by considering the spectral unmixing method of vegetation endmember variability. This paper takes the LAI scale effect as an example. The effectiveness of the method is verified by the UAV(Unmanned Aerial Vehicle) image experiment. Three sub-images were selected, and then they were resampled to two different levels of spatial resolution for experimentation. Among them, the exponential function fitting is performed through the simulated spectrum of the PROSAIL model, and the relationship model is constructed to invert the LAI. The experimental results show that: (1) The spectral unmixing method that uses the PROSAIL model to describe the variability of vegetation endmembers can obtain higher unmixing accuracy; (2) After using this spectral unmixing method, the root mean square error (RMSE) of the LAI scale effect is significantly reduced, and it has a certain effect on the correction of the LAI scale effect. Which can improve the remote sensing scale effect problem to a certain extent. In summary, the spectral unmixing method has a certain effect on the correction of LAI scale differences, and can improve the problem of remote sensing scale effect to a certain extent. However, the research has the following issues that need further consideration: (1) This article only considers the vegetation-soil binary scene, but this method has not verified the multiple endmember scene. (2) The variability of soil and other background endmembers can be further considered. (3) The model can be further optimized to reduce the difference between the PROSAIL model spectrum and the real image spectrum, thereby improving the accuracy of unmixing. (4) In this paper, the evaluation is carried out by the method of upscaling. In the future, more complicated practical factors can be considered for evaluation. At the same time, real images collected at different flight altitudes can also be used for evaluation.